How to grow bacteria on grapes using TSA plates

How to grow bacteria on grapes using TSA plates

Materials:

  • Swab
  • TSA plates
  • Purified water
  • Organic grapes
  • Bunsen burner
  • Incubator 
Incubator by me is licensed under CC BY 2.0
Colonies by me is licensed under CC by 2.0
Set up by me is licensed under CC BY 2.0

 

 

 

 

 

 

 

 

 

 

 

To look at the type of bacteria that’s on a grape, we can grow them and extract their DNA after obtaining a pure culture of the bacteria to study. To start, keep a bunsen burner on near you when culturing plates. Now, take a clean swab, dip it in purified water and rub it all over the grape. Now, using that same swab, rub it on a TSA plate in a back and forth motion to cover the plate; make sure to use the same side you rubbed all over the grape. After swabbing the plates, placed them agar side up in an incubator of 28 degrees to grow. The plates have to be agar side up to ensure that any residue that forms will fall on the lid and not on the growing bacteria. 

 

Photo by me is licensed under CC BY 2.0


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