How to do simple crystal violet staining

How to do simple crystal violet staining

Materials:

  • 2 Sterile loop
  • 1 TSA plates
  • 1 Bunsen burner
  • 1 Close pin
  • 2 Slide
  • 1 DI Water
  • 1 Crystal violet
  • 1 Microscope
  • 1 Slide warmer
  • 1 plastic container
Bacteria by me is licensed under CC BY 2.0
Colonies by me are licensed under CC BY 2.0

 

 

Set up by me is licensed under CC BY 2.0
Slide warmer by me is licensed under CC BY 2.0

 

 

 

 

 

 

 

 

 

To begin simple crystal violet staining, add a drop of DI water on the slides. Then with a sterile loop, loop some of the bacteria on the TSA plate and mix it in with the water on the slides; make sure to spread the liquid to dry it faster and use a different loop for each slide. Now, allow the slides to dry on the slide warmer. When the slides are dry, using a close pin, pass the slide through the fire using a bunsen burner about 3 times quickly. Placed the slides in a plastic container now and drop a few crystal violets stain on them; enough to cover them and wait 1 min. After a min, using DI water, wash off the slides and placed them on a paper towel to dry; lightly damp the top of the slide to dry but not too hard to whip off the bacteria. After the slides are stained purple, look at them under a microscope. 

 

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