Day: April 12, 2018

How to do simple crystal violet staining

How to do simple crystal violet staining

Materials: 2 Sterile loop 1 TSA plates 1 Bunsen burner 1 Close pin 2 Slide 1 DI Water 1 Crystal violet 1 Microscope 1 Slide warmer 1 plastic container                       To begin simple crystal violet staining, 

How to grow bacteria on grapes using TSA plates

How to grow bacteria on grapes using TSA plates

Materials: Swab TSA plates Purified water Organic grapes Bunsen burner Incubator                        To look at the type of bacteria that’s on a grape, we can grow them and extract their DNA after obtaining a pure 

How to make LBS Agar medium

How to make LBS Agar medium

Medium: LBS Agar Approximate Formula* 500mL Ingredients:  Pancreatic Digest of Casein ………………………………… 5.0 g Yeast Extract ……………………………………………………… 2.5 g Monopotassium Phosphate………………………………….. 3.0 g Ammonium Citrate …………………………………………….. 1.0 g Dextrose …………………………………………………………. 10.0 g Polysorbate 80 ……………………………………………………20 drops Sodium Acetate Hydrate ……………………………………. 12.5 g Magnesium Sulfate …………………………………………. 

How to do DNA extraction

How to do DNA extraction

Materials Sterile loop PBS buffer AW1 buffer AW2 buffer AE buffer  Proteinase K Ethanol Tube racks Pipette Pipette tips Microfuge tube Spin column Centrifuge Trash bucket     To perform DNA extraction on a pure colony, collect a tiny amount of cells from the pure colony using 

How to gram stain

How to gram stain

Materials DI water Sterile loop Slide Warmer Bunsen Burner Close pin  Crystal Violet Gram Iodide Ethonal Safranin Slides Plastic container Trash bucket                 To gram stained a culture, pour a drop of DI water on a slide. Then