How to do simple crystal violet staining
Materials:
- 2 Sterile loop
- 1 TSA plates
- 1 Bunsen burner
- 1 Close pin
- 2 Slide
- 1 DI Water
- 1 Crystal violet
- 1 Microscope
- 1 Slide warmer
- 1 plastic container
To begin simple crystal violet staining, add a drop of DI water on the slides. Then with a sterile loop, loop some of the bacteria on the TSA plate and mix it in with the water on the slides; make sure to spread the liquid to dry it faster and use a different loop for each slide. Now, allow the slides to dry on the slide warmer. When the slides are dry, using a close pin, pass the slide through the fire using a bunsen burner about 3 times quickly. Placed the slides in a plastic container now and drop a few crystal violets stain on them; enough to cover them and wait 1 min. After a min, using DI water, wash off the slides and placed them on a paper towel to dry; lightly damp the top of the slide to dry but not too hard to whip off the bacteria. After the slides are stained purple, look at them under a microscope.
