Microbial Diversity

Materials: 2 Sterile loop 1 TSA plates 1 Bunsen burner 1 Close pin 2 Slide 1 DI Water 1 Crystal violet 1 Microscope 1 Slide warmer 1 plastic container                       To begin simple crystal violet staining, …

Materials: Swab TSA plates Purified water Organic grapes Bunsen burner Incubator                        To look at the type of bacteria that’s on a grape, we can grow them and extract their DNA after obtaining a pure …

Medium: LBS Agar Approximate Formula* 500mL Ingredients:  Pancreatic Digest of Casein ………………………………… 5.0 g Yeast Extract ……………………………………………………… 2.5 g Monopotassium Phosphate………………………………….. 3.0 g Ammonium Citrate …………………………………………….. 1.0 g Dextrose …………………………………………………………. 10.0 g Polysorbate 80 ……………………………………………………20 drops Sodium Acetate Hydrate ……………………………………. 12.5 g Magnesium Sulfate …………………………………………. …

Materials Sterile loop PBS buffer AW1 buffer AW2 buffer AE buffer  Proteinase K Ethanol Tube racks Pipette Pipette tips Microfuge tube Spin column Centrifuge Trash bucket     To perform DNA extraction on a pure colony, collect a tiny amount of cells from the pure colony using …

Materials DI water Sterile loop Slide Warmer Bunsen Burner Close pin  Crystal Violet Gram Iodide Ethonal Safranin Slides Plastic container Trash bucket                 To gram stained a culture, pour a drop of DI water on a slide. Then …

Materials TSA powder Agar powder 1L bottle Purified water Stir plate Stir bar Scale Plastic containers                       To make a 1.5% TSA plate, obtain a 1-liter bottle to add the ingredients in. Then used a scale …